International Journal of Biotechnology Research Vol. 2(6), pp. 075-088, October 2014 ISSN 2328-3505 ©2014 Academe Research Journals

Full Length Research Paper

Assessment of genetic purity of some hybrid rice parental lines using protein profile and fertility restorer gene linked markers

Ola A. Galal¹*, Mahmoud I. Abo-Youssef², Mona Abdelaziz³, Ahmed T. Gharib³ and Said A. Dora¹

¹Genetics Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt.

²Rice Research and Training Centre, Agricultural Research Center, Sakha, Kafr El-Sheikh, Egypt.

³Laser Applications in Biotechnology and Agriculture, National Institute of Laser Enhanced Sciences, Cairo University, Egypt.

*Corresponding author. E-mail: ola.galal@agr.kfs.edu.eg, olagalal2002@yahoo.com. Tel/Fax: +2-0479102930.

Accepted 23 October, 2014

Abstract

For identification of rice hybrids and their parental lines, thirty populations produced from hybridization between a cytoplasmic male sterility (CMS) line (IR69625A), and each of the three restorer lines (Giza178R, Giza181R and Giza182R) during 2009, 2010 and 2011 seasons were used. Electrophoresis of proteins showed promising results in genetic purity determination of hybrids and parental lines. The presence or absence of a specific band was much useful for differentiating the highest from the lowest parents in restoring ability. Five markers linked to fertility restorer genes: M2, RM3425, RM258, RM1108 and RM5373 were screened on DNA templates from the CMS line and its three restorer lines. Only RM1108 marker was able to show polymorphism between the highest and lowest Giza181R parents. This marker could be assessed for identification and testing of seed genetic purity of restorer lines.

Key words: Hybrid rice, genetic purity, SDS-PAGE, fertility restorer genes.