International Journal of Biotechnology Research Vol. 3(2), pp. 028-034, February 2015 ISSN 2328-3505 ©2015 Academe Research Journals

Full Length Research Paper

Induction of different lignocellulolytic enzymes in the process of degradation of agricultural waste substrates by the Jew’s ear mushroom

Ahila Devi P.*, Veeralakshmi S., Prakasam V. and Thiribhuvanamala G.

Tamil Nadu Rice Research Institute, Aduthurai – 612 101, Tamil Nadu, India.

*Corresponding author. E-mail: ahila.devi1@gmail.com.

Accepted 22 January, 2015

Abstract

The enzyme potential of different mushrooms varied differently and differed greatly based on the nature of the substrates used for cultivation. But in common, all mushrooms produce both cellulases and laccase, more particularly, for easy degradation and utilization of the substrate. High level of cellulolytic enzyme activities namely: exo β-1, 4 glucanase and endo β-1,4 glucanase was observed in beds prepared with paddy straw + wheat bran (3:1), whereas high level of lignolytic enzyme activity namely, laccase was observed in paddy straw + wheat bran (1:1). Protein profile studies of Auricularia polytricha were compared with those of Pleurotus platypus, Pleurotus florida, and Pleurotus eous. SDS - PAGE analysis of mycelium of different mushrooms revealed the presence of 12-14 protein bands in A. polytricha. Common protein bands were expressed at 14 kDa, 25 kDa and 49 kDa in all the mushrooms.

Key words: Auricularia polytricha, bioefficiency, cellulases, laccase, protein.