International Journal of Biotechnology Research Vol. 3(2), pp. 021-027, February 2015 ISSN 2328-3505 ©2015 Academe Research Journals

Full Length Research Paper

Estimation of genetic variability in Trigonella cultivars by RAPD analysis

Vaseem Raja¹*, Umer Majeed Wani¹, Rajdeep Kudesia² and Riffat John¹

¹Department of Botany, University of Kashmir, India.

²Department of Botany, Bundelkhand University, Jhansi, U.P. India.

*Corresponding author. E-mail: wrajamp2009@gmail.com.

Accepted 19 December, 2014

Abstract

Trigonella is receiving great importance due to its rear medicinal properties. Gene banks harbor scanty germplasm. The neglected use of this important herb had received less attention due to which very little information is available about its genetic diversity. As such, an attempt was made to study the genetic diversity of this important plant. For the present study, five cultivars of Trigonella were analyzed to estimate genetic diversity by using eleven random primers. A total of 80 bands were detected, 66 of which were polymorphic with a polymorphism of 82.50%. The amplified DNA fragments ranged in size from 200-3060 bp. Jaccard’s similarity coefficient ranged from 0.266 to 0.615. A dendrogram constructed based on UPGMA revealed two clusters. Cluster 1 consists of three accessions - T3, T1 and T2, while cluster 2 consists of two accessions - T4 and T5. It is evident from the dendrogram that accession T3 and T5 are genetically diverse; hence it is recommended that these should be used for future breeding programs to create higher amount of genetic variability in Trigonella.

Key words: Trigonella, genetic diversity, polymorphism, dendrogram, monomorphic.