Academe Research Journals

International Journal of Biotechnology Research

Full Length Research Paper

Evaluation of Proximate Analysis, Lycopene Content And Dna Concentration Using Different Dna Extraction Protocols In Tomato Genotypes, Lycopersicon Esculentum (Mill.)

Adewusi, O.F

Department Of Biotechnology Federal University of Technology, Akure, Ondo–State, Nigeria.

Corresponding author. E-mail: ofadewusi@gmail.com. Tel. 08101109482

Accepted September 21, 2024

Abstract

This study was carried out on the “Evaluation of proximate analysis, lycopene content andconcentration of DNA sample usingdifferent DNA extraction protocols in Tomato Genotypes, Lycopersicon esculentum (Mill)”.The study aimed to determine the extent of variation in the level of lycopene and proximate composition and the concentration of DNA samples in the tomato genotypes using different DNA methods among the tomato genotypes with a view of identifying and selecting promising tomato genotypes that could be utilized for further tomato breeding program. The twelvetomato genotypes used for this study were obtained from the Tomato Germplasm collection of the National Centre for Genetic Resources and Biotechnology (NACGRAB), Department of Plant Genetic Resources, Ibadan, Oyo-State, Nigeria. The experiment was conductedat the Teaching and Research Farm Obakekere, Federal University of Technology, Akure. The DNA extraction was carried out using Cetyltrimethylammonium bromide (CTAB), Sodium dodecyl sulfate (SDS), and the Zymo Kit protocol. The concentration and purity levels of the DNA samples were determined using Nano-drop (ND)-2000 spectrophotometer and 1% w/v agarose gel stain with ethidium bromide. The moisture content values were generally high for the genotypes ranging from 78.72-82.46% being the maximum in NGB00713 (82.46%). Genotypes, NGB00695, NGB00696, NGB00725 and NGB00752 recorded lower levels of moisture content. This indicates that these tomato genotypes can remain in store for an extended period compared to the other tomato genotypes. The crude protein content values ranged between 3.60% and 7.45% being the maximum in NGB00726 (7.45%). The carbohydrate content also varied between 4.77% and 10.63% being maximum in NGB00737 (10.63%). The lycopene content ranged between 2.49% and 31.20%. Genotypes, NGB00695, NGB00696, NGB00721 and NGB00735 recorded high levels of lycopene content compared to the other tomato genotypes which implies that they could be selected for improvement in future tomato breeding programs. The CTAB DNA extraction method recorded the concentration and purity levels ranging between 60.00 and 350.40 ng/µl and 1.318 and 2.153 respectively. The concentration and purity level of the Zymo kits ranged between 66.00 and 470.80 ng/µl and 1.728 and 2.210 respectively. For the SDS DNA extraction method, the concentration ranged between 57.600 – 999.600 ng/µl and 1.480 – 2.573 for the purity respectively. The results from the three DNA extraction methods imply that Zymo kits is the best for DNA extraction method in terms of DNA quality whereas SDS is the best in terms of DNA concentration that can be utilized for the extraction of DNA from tomato genotypes.

Keywords: Lycopene, Proximate, Sds, Ctab, Zymo Kits, Dnaconcentration, Tomato Genotypes