International Journal of Microbiology and Immunology Research Vol. 5(1), pp. 001-006, January 2017 ISSN 2327-7769 ©2017 Academe Research Journals

Full Length Research Paper

Prevalence of hepatitis virus delta infection among HBVAgs positive blood donors at the Regional Blood Transfusion Center of Ouagadougou, Burkina Faso

Moutanou Modeste Judes ZEYE^1,2,3*, Serge Potiandi DIAGBOUGA^1,2,4, Florencia Wendkuuni DJIGMA^1,2, Mahamoudou SANOU^5,6, Dorcas Obiri-Yeboah⁷, Serge Yannick OUEDRAOGO^1,2, Abel Pegdwendé SORGHO^1,2, Abdoul Karim OUATTARA^1,2, Arsène Wendpagnangdé ZONGO^1,2, Abdoulaye TANGARA³, Tegwindé Rébéca COMPAORE^1,2, Siaka OUATTARA⁶, Jacques SIMPORE^1,2

¹Laboratory of Molecular Biology and Genetics (LABIOGENE), University Ouaga I Prof. Joseph Ki-Zerbo, P.O. Box 7021, Ouagadougou 03, Burkina Faso.

²Pietro Annigoni Biomolecular Research Center (CERBA), P.O. Box 364, Ouagadougou 01, Burkina Faso.

³Action Preventive Health Association/Center for information and screening on Immunization, Ouagadougou, Burkina Faso.

⁴Research Institute for Health Sciences (IRSS), PO Box 7192, Ouagadougou, Burkina Faso.

⁵Teaching University Pediatric Hospital Charles De Gaulle, Ouagadougou, Burkina Faso.

⁶Regional blood transfusion center, Ouagadougou, Burkina Faso.

⁷Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, PMB, Cape Coast, Ghana.

*Corresponding author. E-mail: jacques.simpore@yahoo.fr.

Accepted 3 January, 2017

Abstract

Background: Infection with hepatitis viruses constitutes a public health problem in Burkina Faso. Several previous studies have estimated the prevalence of viral hepatitis A, B and C. This study aimed to study the prevalence of hepatitis delta infection in apparent healthy blood donors positive for the antigen of hepatitis B virus.

Methods: This study involved first time blood donors, positive for HBV surface antigen (HBsAg). The presence of HBV and HDV was confirmed using real time PCR duplex. The SPSS software was used for statistical analyzes of the obtained results.

Results: Two hundred HBsAg positive blood donors were enrolled in this study. The socio-demographic data showed 79% of men from all religious and ethnic denominations. RT-PCR analysis confirmed the presence of HBV DNA in 195/200 (97.5%) positive blood donors. Only 0.5% (1/200) of HBV positive person presented a case of co-infection with HDV.

Conclusion: Our study shows that 97.5% of HBsAg positive person detected by the ARCHITECT i 1000 SRTM were positive by PCR. The obtained results showed that the distribution of HDV in blood donors positive for the hepatitis B antigen is low (0.5%).

Key words: HBV, HDV, Real Time PCR, Blood donors, Ouagadougou, Burkina Faso.