International Journal of Microbiology and Immunology Research
International Journal of Microbiology and Immunology Research Vol. 5(1), pp. 001-006, January 2017 ISSN 2327-7769 ©2017 Academe Research Journals
Full Length Research Paper
Prevalence of hepatitis virus delta infection among HBVAgs positive blood donors at the Regional Blood Transfusion Center of Ouagadougou, Burkina Faso
Moutanou Modeste Judes ZEYE1,2,3*, Serge Potiandi DIAGBOUGA1,2,4, Florencia Wendkuuni DJIGMA1,2, Mahamoudou SANOU5,6, Dorcas Obiri-Yeboah7, Serge Yannick OUEDRAOGO1,2, Abel Pegdwendé SORGHO1,2, Abdoul Karim OUATTARA1,2, Arsène Wendpagnangdé ZONGO1,2, Abdoulaye TANGARA3, Tegwindé Rébéca COMPAORE1,2, Siaka OUATTARA6, Jacques SIMPORE1,2
1Laboratory of Molecular Biology and Genetics (LABIOGENE), University Ouaga I Prof. Joseph Ki-Zerbo, P.O. Box 7021, Ouagadougou 03, Burkina Faso.
2Pietro Annigoni Biomolecular Research Center (CERBA), P.O. Box 364, Ouagadougou 01, Burkina Faso.
3Action Preventive Health Association/Center for information and screening on Immunization, Ouagadougou, Burkina Faso.
4Research Institute for Health Sciences (IRSS), PO Box 7192, Ouagadougou, Burkina Faso.
5Teaching University Pediatric Hospital Charles De Gaulle, Ouagadougou, Burkina Faso.
6Regional blood transfusion center, Ouagadougou, Burkina Faso.
7Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, PMB, Cape Coast, Ghana.
*Corresponding author. E-mail: firstname.lastname@example.org.
Accepted 3 January, 2017
Background: Infection with hepatitis viruses constitutes a public health problem in Burkina Faso. Several previous studies have estimated the prevalence of viral hepatitis A, B and C. This study aimed to study the prevalence of hepatitis delta infection in apparent healthy blood donors positive for the antigen of hepatitis B virus.
Methods: This study involved first time blood donors, positive for HBV surface antigen (HBsAg). The presence of HBV and HDV was confirmed using real time PCR duplex. The SPSS software was used for statistical analyzes of the obtained results.
Results: Two hundred HBsAg positive blood donors were enrolled in this study. The socio-demographic data showed 79% of men from all religious and ethnic denominations. RT-PCR analysis confirmed the presence of HBV DNA in 195/200 (97.5%) positive blood donors. Only 0.5% (1/200) of HBV positive person presented a case of co-infection with HDV.
Conclusion: Our study shows that 97.5% of HBsAg positive person detected by the ARCHITECT i 1000 SRTM were positive by PCR. The obtained results showed that the distribution of HDV in blood donors positive for the hepatitis B antigen is low (0.5%).
Key words: HBV, HDV, Real Time PCR, Blood donors, Ouagadougou, Burkina Faso.
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