International Journal of Agricultural Science Research Vol. 4(1), pp. 021-023, January 2015 ISSN 2327-3321 ©2015 Academe Research Journals

Short Communication

Adaption of Infectious Bursal Disease Virus (IBDV) on Chicken Embryo-fibroblast Culture

Tariq Mehmood Butt, Rehan Rafique*, Umber Rauf, Muhammad Najiullah khan, Asaad Nayyer, Yasmeen Abbas and Talha Farooq

Veterinary Research Institute, Zarrar Shaheed Road, Lahore Cantt. Punjab, Pakistan.

*Corresponding author. E-mail: dr.rehan702@gmail.com. Tel: +92-333-6607756. Zip Code/Postal Code 54810.

Accepted 24 November, 2014

Abstract

Infectious bursal disease virus (IBDV) from the local outbreak in poultry flocks was purified and confirmed through Agar gel immune-diffusion test (AGID). Purified IBD virus was attenuated by serial passages in eggs through chorioallantoic membrane (CAM) route. The mortality was up to 70% in the first 6 passages and reduced from 60 to 0% up to the 16th passage. No mortality was observed between 16th-24th passages. Chicken embryo fibroblasts were prepared from 9 days-old specific pathogen free (SPF) embryonating eggs and then infected with purified IBDV. Typical cytopathic effects (initiated from star-shaped cells which led to rounding of fibroblast cells) were observed on inverted microscope. This approach will help to produce cost effective live-attenuated infectious bursal disease vaccine from local strains on chicken embryo fibroblasts cells.

Key words: Infectious bursal disease virus (IBDV), Agar gel immunodiffusion test (AGID), chorioallantoic membrane, chicken embryo fibroblast, specific pathogen free (SPF).